Glutathione - WikiMili, The Free Encyclopedia.
The widely used glutathione redox potential sensor Grx1-roGFP2 was developed by the fusion of redox sensitive roGFP2 with the human glutaredoxin-1 (Grx1) domain to improve the specificity of roGFP2 toward reporting the glutathione redox state (Gutscher et al., 2008). The sensor exhibits excitation maxima at 408 nm for oxidized Grx1-roGFP2 and 488 nm for reduced Grx1-roGFP2, with emission at.
GSH and GSSG levels were measured by NMR for both cells at rest and under oxidative stress, and then used to calculate the changing intracellular redox potentials over time. The results showed, for the first time ever, that redox potential can serve as an indicator of how cells perform in response to oxidation in real time.
Real-time imaging of the intracellular glutathione redox potential in the malaria parasite Plasmodium falciparum. 3-morpholino-sydnonimine hydrochloride: Real-time imaging of the intracellular glutathione redox potential in the malaria parasite Plasmodium falciparum. 7-benzylidenenaltrexone: Profiling molecular factors associated with pyknosis and developmental arrest induced by an opioid.
In this regard, we have implemented a ratiometric, genetically encoded redox-sensitive green fluorescent protein fused to human glutaredoxin (Grx1-roGFP2) to monitor real-time glutathione redox potentials in the cytosol and mitochondrial matrix of tumorigenic and non-tumorigenic cells. First, we demonstrated that recovery time in both compartments depended upon the length of exposure to.
Jun 07, 2011: Development of a FRET sensor for real-time imaging of intracellular redox dynamics (Nanowerk News) In work published in the June 2011 issue of Experimental Biology and Medicine, Kolossov, Spring and their co-investigators - a multidisciplinary team within the Institute for Genomic Biology at the University of Illinois - have transferred the concept of redox-sensitive Green.
Real-Time Imaging of the Intracellular Glutathione Redox Potential in the Malaria Parasite Plasmodium falciparum PLoS Pathogens, Dec 2013 Denis Kasozi, Franziska Mohring, Stefan Rahlfs, Andreas J. Meyer, Katja Becker.
Real-time imaging of the intracellular glutathione redox potential. Nat Methods 5:553-559. Nat Methods 5:553-559. Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, et al. 2004.